Transcriptome sequencing is a method used to analyze all the RNA transcripts present in a cell or tissue at a specific time. This technique allows researchers to study gene expression levels, identify differentially expressed genes, and gain insights into the biological processes occurring within a cell or organism. By sequencing the transcriptome, researchers can obtain a comprehensive view of the gene expression patterns and regulatory mechanisms underlying various biological processes, such as development, disease, and response to external stimuli. This information can help to unravel the complexity of gene regulation and provide valuable insights into the functioning of cells and organisms.